When Fluorescence Misleads: Building Better Antibody-Based Assays...

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2026-10-19 08:38:35 -

When Fluorescence Misleads: Building Better Antibody-Based Assays for Natural Compounds

A brighter image does not always mean more target protein, and a weaker signal does not necessarily demonstrate inhibition. In experiments involving natural compounds, the detection chemistry can change independently of the biology. When fluorescent antibodies are used to measure cellular responses, reliable interpretation depends on three connected choices: the test compound, the antibody...

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